Jun Hong Park, Kyung In Cho, Hajin Nam, Nong-Hoon Choe, Jun Gyo Suh.
/
Animal Cells and Systems.
/
제 19권
3호
/
2015
Silk fibroin
/
Silk fibroin
Hyperglycemia-induced pancreatic β-cell apoptosis causes serious health complications in diabetic patients. Recently, studies demonstrated that silk and silk-related materials have anti-diabetic effects. We previously reported that silk fibroin hydrolysate (SFH) has anti-diabetic effects through increased pancreatic β-cell mass in type 2 diabetic animals (C57BL/KsJdb/db). However, it is not known whether SFH has anti-apoptotic effects in hyperglycemic conditions. The present study investigates the anti-apoptotic effects of SFH on high glucose-induced apoptosis using RIN5F cells, a rat pancreatic β-cell line. Hyperglycemic conditions decreased RIN5F cell viability in a concentration-dependent manner. High glucose treatment of 33 mM or higher caused a significant decrease in RIN5F cell viability. However, addition of SFH significantly recovered cell viability in the presence of high glucose. Flow cytometry analysis showed that high glucose treatment significantly increased early stage apoptosis in RIN5F cells. This was inhibited by SFH treatment, which significantly decreased not only early stage apoptosis but also decreased the production of nitrite. Additionally, SFH protected RIN5F cells from oxidative stress-induced apoptosis. These results suggest that SFH has anti-apoptotic effects by protecting pancreatic β-cell from high glucose and/or oxidative stress. Our results support in vivo anti-diabetic effects of SFH and validation of the traditional use of silkworm and silkworm materials in the treatment of diabetes.
송준호, 양선규, 김욱진, 문병철
/
한약정보연구회지
/
제 7권
1호
/
2019
선퇴(蟬退)
/
말매미
약재로 유통되고 있는 충부약재 선퇴의 기원종, 말매미에 대한 정확하고 자세한 정보를 제시하고자 하였다.
1. 말매미의 정명, 이명을 정리하고, 형태학적 감별을 위한 정성적, 정량적 형질을 자세히 기재하였다.
2. 암수 성간 정량적 형질의 비교분석 결과, 두 성간 유의미한 차이가 확인되지 않았다.
3. 생식기의 형태로 암수가 구별 가능하였고, 생식기 주변부의 미세형태학적 형질 역시 차이가 나타나지 않았다.